adipogenesis marker antibody sampler kit  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Hepatocyte and adipocyte CDO1-mediated intracellular cysteine catabolism differentially modulates diet-induced obesity and fatty liver in mice

doi: 10.64898/2025.12.05.692669

Figure Lengend Snippet: A. Cysteine metabolism pathways. CoA: coenzyme A. FAO: fatty acid oxidation. B. Liver CDO1 protein. C, D. Liver metabolites. n=4-5. Mice were fasted overnight (fast) or fed chow for 6 hours after overnight fasting (fed). E-H. Mice were fed chow or WD for 16 weeks. n=5-8. Body weight (E), body composition (F, G), and representative of immunohistochemistry stain of CDO1 in liver section. Scale bar = 250 μm. All results are shown as mean ± SEM.

Article Snippet: Anti-CDO1 antibody (12589-1-AP) was purchased from Proteintech Group, Inc. (Rosemont, IL).

Techniques: Immunohistochemistry, Staining

Journal: bioRxiv

Article Title: Hepatocyte and adipocyte CDO1-mediated intracellular cysteine catabolism differentially modulates diet-induced obesity and fatty liver in mice

doi: 10.64898/2025.12.05.692669

Figure Lengend Snippet: Mice were fed HFCFr diet for 8 months. A. Body weight. n=11-15. B-C. Body composition. D. Blood glucose after 6 hours fasting measured during the 8 th month of HFCFr diet feeding. E. Liver weight (LW) to body weight (BW) ratio. F-G. Blood aspartate aminotransferase (AST) and alanine aminotransferase (ALT). H. Liver CDO1 protein after 8 months HFCFr diet feeding. I. Liver triglycerides (TG). J. Liver total cholesterol. K. Liver H&E stain. Scale bar = 250 μm. L. Liver Sirius red stain. Upper panels, 4x view, Scale bar = 600 μm. Lower panels, 10 x view, Scale bar = 250 μm. All results are shown as mean ± SEM.

Article Snippet: Anti-CDO1 antibody (12589-1-AP) was purchased from Proteintech Group, Inc. (Rosemont, IL).

Techniques: Staining

Journal: bioRxiv

Article Title: Hepatocyte and adipocyte CDO1-mediated intracellular cysteine catabolism differentially modulates diet-induced obesity and fatty liver in mice

doi: 10.64898/2025.12.05.692669

Figure Lengend Snippet: A. Liver CDO1 protein in mice fed WD for 2 weeks. B-C. Liver metabolites in mice fed WD for 2 weeks. n=5. D-H. Mice were fed chow or WD for 16 weeks. n=6-11. Body weight (D), body composition (E), LW:BW ratio (F), liver H&E stain, Scale bar = 250 μm (G), and 6 hours fasting blood glucose. All results are shown as mean ± SEM.

Article Snippet: Anti-CDO1 antibody (12589-1-AP) was purchased from Proteintech Group, Inc. (Rosemont, IL).

Techniques: Staining

Journal: bioRxiv

Article Title: Hepatocyte and adipocyte CDO1-mediated intracellular cysteine catabolism differentially modulates diet-induced obesity and fatty liver in mice

doi: 10.64898/2025.12.05.692669

Figure Lengend Snippet: A. CDO1 protein in liver, epidydimal white adipose tissue (eWAT), and brown adipose tissue (BAT) of chow-fed mice. B-D. Mice were fed WD for 16 weeks. n=7-17. Body weight (B), eWAT H&E stain, Scale bar = 250 μm (C), and BAT H&E stain, Scale bar = 600 μm (D). BW results are shown as mean ± SEM.

Article Snippet: Anti-CDO1 antibody (12589-1-AP) was purchased from Proteintech Group, Inc. (Rosemont, IL).

Techniques: Staining

Journal: bioRxiv

Article Title: Hepatocyte and adipocyte CDO1-mediated intracellular cysteine catabolism differentially modulates diet-induced obesity and fatty liver in mice

doi: 10.64898/2025.12.05.692669

Figure Lengend Snippet: A. CDO1 protein in liver, epidydimal white adipose tissue (eWAT), and brown adipose tissue (BAT) of chow-fed mice. B-F. Mice were fed chow or WD for 16 weeks. n=4-8. Body weight (B), body composition (C, D), and representative H&E stain of eWAT, BAT, and liver sections, Scale bar = 250 μm (E), and liver weight (F). All results are shown as mean ± SEM.

Article Snippet: Anti-CDO1 antibody (12589-1-AP) was purchased from Proteintech Group, Inc. (Rosemont, IL).

Techniques: Staining

Journal: Nature

Article Title: Taurine from tumour niche drives glycolysis to promote leukaemogenesis

doi: 10.1038/s41586-025-09018-7

Figure Lengend Snippet: a , Circos plot showing leukaemia cell surface receptors and cognate stromal-cell-derived ligands. b , Kaplan–Meier curves of human patients with leukaemia with low (<11.14, n = 80) or high (≥11.14, n = 81) SLC6A6 expression (TCGA-LAML; Xena Browser; log-rank test). CI, confidence interval; HR, hazard ratio. c , Normalized SLC6A6 expression in CD43 + cells from normal human bone marrow (BM) samples and samples from patients with bcCML and AML. n = 7 (bone marrow), n = 10 (bcCML) and n = 11 (AML). For the box plots, the centre line shows the median, the box limits show the interquartile range and the whiskers represent the minimum and maximum values, respectively. Statistical analysis was performed using DESeq2-implemented Wald tests. d – g , Representative IHC images ( d , f ) and quantification ( e , g ) of CDO1 expression in matched patient bone marrow biopsies at MDS diagnosis and after AML transformation ( d , e ), or at AML diagnosis (AML-D) and relapse (AML-R) ( f , g ). n = 5 independent patients per cohort. Each colour represents a patient sample. Statistical analysis was performed using two-tailed ratio paired t -tests. h , The strategy used to determine the impact of inhibiting CDO1 in human bone marrow MSCs on co-cultured AML cells (MSCs and AML cells were derived from the same patient). CFU, colony-forming unit. i , The number of live LSCs (left; data are mean ± s.e.m.; n = 11 independent culture wells per cohort; data were combined from two independent experiments) and their colony-forming ability (right; data are mean ± s.d.; n = 3 independent culture wells per cohort) after coculture with AML MSCs. j , Taurine quantity per femur in control and leukaemic mice, as determined using colourimetric analysis, 12 days after transplant. Data are mean ± s.e.m. n = 5 animals per cohort. Data were combined from two independent experiments. For i and j , statistical analysis was performed using unpaired two-tailed Student’s t -tests. k , Experimental strategy and survival curve, showing the impact of blocking taurine production by MSC/osteolineage populations in vivo on bcCML progression in unirradiated recipients. n = 18 ( Cdo1 fl/fl / Cdo1 +/+ ) and n = 14 ( Cdo1 fl/fl Prrx1-cre + ). Data were combined from four independent experiments. Statistical analysis was performed using the log-rank test. WT, wild type. Scale bars, 50 µm ( d and f ). The mouse images in h and k are adapted from ref. , Springer Nature America.

Article Snippet: Primary antibodies used included mTOR (Cell Signaling Technologies), LAMP1 (DHSB) or CDO1 (Proteintech).

Techniques: Derivative Assay, Expressing, Biomarker Discovery, Transformation Assay, Two Tailed Test, Cell Culture, Control, Blocking Assay, In Vivo

Journal: Nature

Article Title: Taurine from tumour niche drives glycolysis to promote leukaemogenesis

doi: 10.1038/s41586-025-09018-7

Figure Lengend Snippet: a , Kaplan-Meier curves of human leukaemia patients with high (<9.32, n = 80) or low (≥9.32, n = 81) LDLR expression (TCGA-LAML; Xena Browser; log-rank test). b , Normalized LDLR expression in CD34 + cells from human bcCML, AML, or normal BM (n = 7 normal BM, n = 10 bcCML, n = 11 AML; central line, box, and whiskers represent median, interquartile range or IQR, minimum/maximum within 1.5*IQR respectively; DESeq2 implemented Wald test). c, d , Experimental strategy ( c ) and relative Apoe expression ( d ) in MSCs transduced with shRNAs targeting LacZ or Apoe (mean ± s.d.; n = 3 technical replicates per cohort). e,f , Number of live leukaemia cells ( e ) and their colony forming ability (CFU) ( f ) post co-culture with MSCs (mean ± s.e.m.; e , n = 8 independent culture wells; f , n = 6 independent culture wells, data combined from two independent experiments; unpaired two-tailed Student’s t-test). g , Slc6a6 expression in KLS cells infected with BCR-ABL and NUP98-HOXA9 (BA-NH) oncogenes for 48 h (mean ± s.d.; n = 3 technical replicates per cohort). h , Taurine biosynthesis pathway. i , Dot plot of Cdo1 and Csad expression during disease progression. j , UMAP plot of CDO1 and CSAD expression in normal human BM . k , UMAP plot of microenvironmental cells in three human MDS or AML BM aspirates. l , UMAP plot of CDO1 and CSAD in human MDS and AML BM aspirates. m - p , Representative IHC images and quantification of Osterix expression in matched human BM biopsies at MDS diagnosis and AML transformation ( m, n ) or AML diagnosis and relapse ( o,p ) (n = 5 independent patients per cohort; each colour represents a patient sample; two-tailed ratio paired t-test). The mouse image in c is adapted from ref. , Springer Nature America.

Article Snippet: Primary antibodies used included mTOR (Cell Signaling Technologies), LAMP1 (DHSB) or CDO1 (Proteintech).

Techniques: Expressing, Transduction, Co-Culture Assay, Two Tailed Test, Infection, Biomarker Discovery, Transformation Assay

Journal: Nature

Article Title: Taurine from tumour niche drives glycolysis to promote leukaemogenesis

doi: 10.1038/s41586-025-09018-7

Figure Lengend Snippet: a, b , Alizarin red staining ( a ), and Cdo1 and Csad expression ( b ) in MSCs undergoing osteogenic differentiation (mean ± s.d.; n = 3 technical replicates per cohort). c , CDO1 expression in murine leukaemia and patient AML MSCs undergoing osteogenic differentiation (mean ± s.e.m.; n = 3 replicates per time point). d , Taurine in MSC culture media during osteogenic differentiation (mean ± s.e.m.; n = 3 replicates; secreted over 48–72 h). e,f , Experimental strategy ( e ) and Cdo1 expression ( f ) in MSCs transduced with shRNAs targeting LacZ or Cdo1 (mean ± s.d.; n = 3 technical replicates per cohort). g , Live leukaemia cells (left), and CFU (right), post coculture with bcCML MSCs (mean ± s.e.m.; n = 7 independent culture wells per cohort (live); n = 6 (CFU); data combined from 2 independent experiments; one-way ANOVA). h , CDO1 expression in 293 T transduced with shRNAs targeting LACZ or CDO1 (mean ± s.d.; n = 3 technical replicates per cohort). i , Cdo1 fl/fl model. j,k , PCR of Cdo1 ( j ) and Cdo1 expression ( k ) in Cdo1 fl/fl and Cdo1 fl/fl ;Prrx1-Cre + MSCs following osteogenic differentiation (mean ± s.d.; n = 3 technical replicates per cohort). l - n , BM stroma in leukaemic control and Cdo1 fl/fl ;Prrx1-Cre + mice: MSCs ( l ), osteolineage cells ( m ), and, endothelial cells ( n ) (mean ± s.e.m.; n = 7 Cdo1 fl/fl and n = 3 Cdo1 fl/fl ;Prrx1-Cre + ; data combined from three independent experiments; unpaired two-tailed Student’s t-test). o-q , CFU of murine cKit + AML cells ( o ), murine Lin − LSCs ( p ), or primary human AML cells ( q ) with taurine (mean ± s.e.m.; n = 3 independent culture wells from n = 2 murine AML, n = 2 murine bcCML, and n = 3 primary human AML samples; data combined from two independent experiments; one-way ANOVA). r , Impact of taurine supplements on murine bcCML progression in unirradiated recipients (n = 14 no taurine and 0.01 mg/ml taurine, n = 15 10 mg/ml taurine; data combined from three independent experiments; log-rank test). The mouse image in e is adapted from ref. , Springer Nature America.

Article Snippet: Primary antibodies used included mTOR (Cell Signaling Technologies), LAMP1 (DHSB) or CDO1 (Proteintech).

Techniques: Staining, Expressing, Transduction, Control, Two Tailed Test